MCB Joint Seminar: Michael Angelo "Spatial organization of human tissue in health and disease"

Assistant Professor, Department of Pathology, Stanford University School of Medicine

"Understanding the role of distinct cellular phenotypes in tissue function, development, and pathogenesis requires tools that can rapidly and consistently quantify the expression of multiple proteins while preserving spatial information. To meet this need, we have developed Multiplexed Ion Beam Imaging by Time-Of-Flight (MIBI-TOF). MIBI-TOF uses secondary ion mass spectrometry to visualize up to 50 metal labeled antibodies at subcellular resolution simultaneously with histochemical stains and native biological elements. We have used this capability to study how single cell function and tissue structure interact at the tumor immune microenvironment, the human maternal-fetal interface, and in infectious disease. These studies have led us to develop scalable, automated, and extensible tools for image thresholding, single cell segmentation, cell clustering, and cell neighborhood analyses that can be deployed on any tissue. We are now extending this platform to incorporate coregistered multimodal studies using MALDI that will link tissue structure to glycan composition and extracellular matrix remodeling."

Zoom Passcode: MCBSeminar

April 19, 2022 - 11:00am to 12:15pm
Event Type: