Timothy Bolger

Associate Professor, Molecular and Cellular Biology
Associate Professor, Genetics - GIDP

Our research in the Bolger lab has dual goals: 1. addressing fundamental biological questions, and 2. utilizing this knowledge to advance human health. The Bolger laboratory uses the budding yeast Saccharomyces cerevisiae as a model system, and takes advantage of the combination of genetics, biochemistry, and cell biology allowed by yeast work. Our long-term goal in fundamental biology is to uncover the regulation of mRNP dynamics. Specifically, we are focusing on the in vivo roles, molecular targets, and regulation of DEAD-box proteins. Presently, we are examining the regulation of Ded1 in translation, both by Gle1 and by post-translational modifications. We are also interested in examining the function and regulation of other DEAD-box proteins. Our research will not only greatly increase our understanding of how these factors function in translation but will elucidate potential mechanisms for control of gene expression, including aiding future studies of functional coupling between mRNA export and translation.

Publications

2021

Brown, N. P., A. M. Vergara, A. B. Whelan, P. Guerra, and T. A. Bolger, "Medulloblastoma-associated mutations in the DEAD-box RNA helicase DDX3X/DED1 cause specific defects in translation.", J Biol Chem, pp. 100296, 2021 Jan 15. PMID: 33460649

2020

Fernandes, N., L. Nero, S. M. Lyons, P. Ivanov, T. M. Mittelmeier, T. A. Bolger, and J.R. Buchan, "Stress Granule Assembly Can Facilitate but Is Not Required for TDP-43 Cytoplasmic Aggregation.", Biomolecules, vol. 10, issue 10, 2020 Sep 25. PMCID: PMC7650667 PMID: 32992901

2019

Aryanpur, P. P., D. M. Renner, E. Rodela, T. M. Mittelmeier, A. Byrd, and T. A. Bolger, "The DEAD-box RNA helicase Ded1 has a role in the translational response to TORC1 inhibition.", Mol Biol Cell, vol. 30, issue 17, pp. 2171-2184, 2019 08 01. PMCID: PMC6743465 PMID: 31141444

2017

Aryanpur, P. P., C. A. Regan, J. M. Collins, T. M. Mittelmeier, D. M. Renner, A. M. Vergara, N. P. Brown, and T. A. Bolger, "Gle1 Regulates RNA Binding of the DEAD-Box Helicase Ded1 in Its Complex Role in Translation Initiation.", Mol Cell Biol, vol. 37, issue 21, 2017 Nov 01. PMCID: PMC5640818 PMID: 28784717

2011

Bolger, T. A., and S. R. Wente, "Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation.", J Biol Chem, vol. 286, issue 46, pp. 39750-9, 2011 Nov 18. PMCID: PMC3220593 PMID: 21949122

2010

Alcázar-Román, A. R., T. A. Bolger, and S. R. Wente, "Control of mRNA export and translation termination by inositol hexakisphosphate requires specific interaction with Gle1.", J Biol Chem, vol. 285, issue 22, pp. 16683-92, 2010 May 28. PMCID: PMC2878036 PMID: 20371601

2008

Bolger, T. A., A. W. Folkmann, E. J. Tran, and S. R. Wente, "The mRNA export factor Gle1 and inositol hexakisphosphate regulate distinct stages of translation.", Cell, vol. 134, issue 4, pp. 624-33, 2008 Aug 22. PMCID: PMC2601711 PMID: 18724935

2007

Tran, E. J., T. A. Bolger, and S. R. Wente, "SnapShot: nuclear transport.", Cell, vol. 131, issue 2, pp. 420, 2007 Oct 19. PMID: 17956740
Bolger, T. A., X. Zhao, T. J. Cohen, C-C. Tsai, and T-P. Yao, "The neurodegenerative disease protein ataxin-1 antagonizes the neuronal survival function of myocyte enhancer factor-2.", J Biol Chem, vol. 282, issue 40, pp. 29186-92, 2007 Oct 05. PMID: 17646162

2005

Zhao, X., T. Sternsdorf, T. A. Bolger, R. M. Evans, and T-P. Yao, "Regulation of MEF2 by histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications.", Mol Cell Biol, vol. 25, issue 19, pp. 8456-64, 2005 Oct. PMCID: PMC1265742 PMID: 16166628
Bolger, T. A., and T-P. Yao, "Intracellular trafficking of histone deacetylase 4 regulates neuronal cell death.", J Neurosci, vol. 25, issue 41, pp. 9544-53, 2005 Oct 12. PMCID: PMC6725694 PMID: 16221865

2001

Zhao, X., A. Ito, C. D. Kane, T. S. Liao, T. A. Bolger, S. M. Lemrow, A. R. Means, and T. P. Yao, "The modular nature of histone deacetylase HDAC4 confers phosphorylation-dependent intracellular trafficking.", J Biol Chem, vol. 276, issue 37, pp. 35042-8, 2001 Sep 14. PMID: 11470791
Fazio, I. K., T. A. Bolger, and G. Gill, "Conserved regions of the Drosophila erect wing protein contribute both positively and negatively to transcriptional activity.", J Biol Chem, vol. 276, issue 22, pp. 18710-6, 2001 Jun 01. PMID: 11278998